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刘耀光

发布者:nxy发布时间:2024-06-19浏览次数:693


         姓        名:刘耀光
         技术职称:研究员
         学位学历:博士研究生
         导师类别:博士生导师、硕士生导师
         联系电话:
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中国科学院院士

华南农业大学一级岗研究员,博士生导师

国家重点人才工程(国内) 入选人

亚热带农业生物资源保护与利用国家重点实验室副主任

华南农业大学作物学一级学科带头人

主要从事植物育性发育的分子遗传机理和基因工程研究。在水稻细胞质雄性不育与恢复性、杂种不育与亲和性、光温敏不育性等遗传系统的基因克隆和分子作用机理方面取得了系统性、创新性研究成果;开发了多项新生物技术发表SCI论文200多篇,论文总引2万余次;获发明专利38项。

 1997年获“国家杰出青年科学基金”,2002年受聘国家重点人才工程(国内),2004年获全国五一劳动奖章,2012年入选广东省“南粤百杰”,2014年获“全国优秀科技工作者”称号,2017年当选中国科学院院士。

 

主要研究领域

水稻分子遗传学、植物基因工程、农作物精准育种创新平台建设等。

 

工作经历

1982.03-1984.08 湖南省长沙市农业专科学校,助教

1991.04-1996.06 日本三井植物生物技术研究所,研究员

1996.07-至今 华南农业大学,研究员

 

科研奖励

1.  云南省自然科学一等奖2021AA006-R007,2022.5.17),稻属AA基因组种间杂种不育遗传研究。完成人:陶大云,万建民,周家武,李静,张玉,赵志刚,刘耀光(陈乐天、谢勇尧

2.  首届广东农业科技创新大比武总决赛-冠军(2021.5.26),高效的作物生物技术精准育种平台的开发与应用。完成人:刘耀光陈乐天,祝钦泷,谢先荣,郭晶心,张群宇,谢勇尧,陈远玲,刘伟智,赵秀彩,初志战

3.  国家科学技术奖励自然科学二等奖(2018-Z-105-2-04-R012018.12.12),杂交稻育性控制的分子遗传基础。完成人:刘耀光,罗荡平,王中华,龙云铭,唐辉武

4.  大北农科技奖基因工程奖(2017-DBNSTA-JYGC-HOLD-N01-01-D01 2017),高效的植物基因组编辑载体系统和软件工具包及其应用。完成人:刘耀光,马兴亮,谢先荣,陈乐天,张群宇,祝钦泷,郭晶心,陈远玲,沈荣鑫,谢勇尧,唐辉武,赵秀彩

5.  大北农科技奖创新奖一等奖(2015-DBNSTA-02-01362015),杂交稻育性控制的分子遗传基础。完成人:刘耀光,王中华,罗荡平,龙云铭,唐辉武,邹艳姣

6.  广东省科学技术奖(自然科学类)一等奖(粤府证[2012]133号,2012, 杂交稻育性控制的分子遗传机理。完成人:刘耀光,王中华,龙云铭,邹艳姣, 张群宇,赵利锋, 李晓瑜, 郭晶心, 陈远玲, 陈乐天, 杨存义,刘振兰, 庄楚雄, 张桂权,梅曼彤

 

学术服务

1.Seed Biology》编委

2. 崖州湾国家实验室和多个全国重点实验室学术委员

 

主持的课题(近十年)

1.   农作物精准育种创新平台建设2023-NJS-00-012省级乡村振兴战略专项种业振兴行动项目,202312月至202411

2.   稻属杂种不育与生殖隔离的分子遗传基础31991220,国家自然科学基金重大项目,20201月至202412

3.   水稻高产优质关键性状的分子机理解析及种质创新2019B030302006,广东省基础与应用基础研究重大项目,20201月至202412

4.   水稻野败型细胞质雄性不育恢复基因Rf4的分子机理与演化201904020030,广州市科技计划重点项目,20194月至20223

5.   水稻穗型发育的分子调控机理研究91435203,国家自然科学基金重点项目,20151月至201712

6.   杂交生殖障碍与育性转换的遗传分子机制2013CB126904,国家重点基础研究发展计划(973 计划),20131月至20178

7.   高产性状分子设计2013CBA01401, 国家重点基础研究发展计划(973 计划),20131月至20178

8.   水稻野败型细胞质雄性不育及其恢复性的分子遗传基础31230052,国家自然科学基金重点项目,20131月至201712

9.   雄性和雌性不育分子机理与杂种优势利用 2011CB100203,国家重点基础研究发展计划(973 计划),20111月至201212

 

论文著作(影响因子大于10近十年发表的论文)

1.  Zheng Y, Zhang S, Luo Y, Li F, Tan J, Wang B, Zhao Z, Lin H, Zhang T, Liu J, Liu X, Guo J, Xie X, Chen L, Liu Y-G*, Chu Z*. Rice OsUBR7 modulates plant height by regulating histone H2B monoubiquitination and cell proliferation. Plant Communications, 2022, 3(6):100412.

2.  Zhao Y, Han J, Tan J, Yang Y, Li S, Gou Y, Luo Y, Li T, Xiao W, Xue Y, Hao Y, Xie X, Liu Y-G*, Zhu Q*. Efficient assembly of long DNA fragments and multiple genes with improved nickase-based cloning and Cre/loxP recombination. Plant Biotechnology Journal, 2022, 20 (10):1983–1995.

3. Zhu Q*, Tan J, Liu Y-G. Molecular farming using transgenic rice endosperm. Trends in Biotechnology, 2022, 40 (10):1248–1260.

4.  Zhao Z#, Xie X #, Liu W# , Huang J, Tan J, Yu H, Zong W, Tang J, Zhao Y, Xue Y, Chu Z, Chen L*, Liu Y-G*. STI PCR: an efficient method for amplification and de novo synthesis of long DNA sequences. Molecular Plant, 2022, 15(4):620–629.

5.  Chen R, Deng Y, Ding Y, Guo J, Qiu J, Wang B, Wang C, Xie Y, Zhang Z, Chen J, Chen L, Chu C, He G, He Z, Huang X, Xing Y, Yang S, Xie D*, Liu Y-G*, Li J*. Rice functional genomics: decades' efforts and roads ahead. Science China Life Sciences, 2022, 65(1):33–92.

6.  Xie X#, Du H#, Tang H, Tang J, Tan X, Liu W, Li T, Lin Z, Liang C*, Liu Y-G*. A chromosome-level genome assembly of the wild rice Oryza rufipogon facilitates tracing the origins of Asian cultivated rice. Science China Life Sciences, 2021, 64 (2):282–293.

7.  Xie X*, Liu W, Dong G, Zhu Q, Liu Y-G*. MMEJ-KO: a web tool for designing paired CRISPR guide RNAs for microhomologymediated end joining fragment deletion. Science China Life Sciences, 64(6), 1021–1024.

8. Wang M, Chen J, Zhou F, Yuan J, Chen L, Wu R,  Liu Y-G*, Zhang Q*. The ties of brotherhood between japonica and indica rice for regional adaptation. Science China Life Sciences, 2021, doi: 10.1007/s11427-021-2019-x.

9. Ma K, Han J, Zhang Z, Li H, Zhao Y, Zhu Q, Xie Y,  Liu Y-G,  Chen L*. OsEDM2L mediates m6A of EAT1 transcript for proper alternative splicing and polyadenylation regulating rice tapetal degradation. Journal of Integrative Plant Biology, 2021,63 (11):1982–1994.

10. Liu T#, Zeng D#, Zheng Z, Lin Z, Xue Y, Li T, Xie X, Ma G, Liu Y-G*, Zhu Q*. The ScCas9++ variant expands the CRISPR toolbox for genome editing in plants. Journal of Integrative Plant Biology, 2021, 63 (9):1611-1619.

11. Zong W, Ren D, Huang M, Sun K, Feng J, Zhao J, Xiao D, Xie W, Liu S,  Zhang H, Qiu R, Tang W, Yang R, Chen H, Xie X, Chen L, Liu Y-G*, Guo J*. Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading. New Phytologist. 2021, 229:1635–1649. doi: 10.1111/nph.16946.

12. Zeng D#, Liu T#, Tan J, Zhang Y, Zheng Z, Wang B, Zhou D, Xie X, Guo M, Liu Y-G*, Zhu Q*. PhieCBEs: Plant High-efficiency Cytidine Base Editors with Expanded Target Range. Molecular Plant, 2020, 13 (12):1666–1669.

13. Zeng D, Liu T, Ma X, Wang B, Zheng Z, Zhang Y, Xie X, Yang B, Zhao Z, Zhu Q*, Liu Y-G*. Quantitative regulation of Waxy expression by CRISPR/Cas9-based promoter and 5'UTR-intron editing improves grain quality in rice. Plant Biotechnology Journal, 2020, 18 (12):2385-2387.

14. Hao Y, Zong W, Zeng D, Han J, Chen S, Tang J, Zhao Z, Li X, Ma K, Xie X, Zhu Q, Chen Y, Zhao X, Guo J*, Liu Y-G*. Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants. Science China-Life Sciences. 2020, 63 (6):933-935.

15. Xie X*, Liu W, Dong G, Zhu Q, Liu Y-G*. MMEJ-KO: a web tool for designing paired CRISPR guide RNAs for microhomology-mediated end joining fragment deletion. Science China-Life Sciences. 2021, 64 (6):1021-1024. DOI: 10.1007/s11427-020-1797-3.

16. Zhao Z, Zhang Z, Ding Z, Meng H, Shen R, Tang H, Liu Y-GChen L*. Public-transcriptome-database-assisted selection and validation of reliable reference genes for qRT-PCR in rice. Science China-Life Sciences, 202063 (1):92-101.

17. Tan J, Zhao Y, Wang B,  Hao Y, Wang Y, Li Y, Luo W, Zong W, Li G, Chen S, Ma K, Xie X, Chen L, Liu Y-G*, Zhu Q*. Efficient CRISPR/Cas9-based plant genomic fragment deletions by microhomology-mediated end joining. Plant Biotechnol J. 2020, 18(11):2161-2163.

18. Zeng D,Li X, Huang J, Li Y, Cai S, Yu W, Li Y, Huang Y, Xie X, Gong Q, Tan J, Zheng Z, Guo M, Liu Y-G*, Zhu Q*. Engineered Cas9 variant tools expand targeting scope of genome and base editing in rice. Plant Biotechnology Journal. 2020, 18(6):1348-1350.

19. Xie Y, Tang J, Xie X, Li X, Huang J, Fei Y, Han J, Chen S, Tang H, Zhao X, Tao D, Xu P, Liu, Y-G, Chen L*. An asymmetric allelic interaction drives allele transmission bias in interspecific rice hybrids. Nat Commun. 2019, 10:2501.  DOI: 10.1038/s41467-019-10488-3.

20. Tang J, Chen L, Liu Y-G*. 2019. Off-target effects and the solution. Nature Plants.5(4):341-342.

21. Zhu Q, Zeng D, Yu S, Cui C, Li J, Li H, Chen J, Zhang R, Zhao X, Chen L, Liu Y-G*. 2018.From Golden Rice to aSTARice: Bioengineering Astaxanthin Biosynthesis in Rice Endosperm. Molecular Plant. 11:1440–1448.

22. Li X, Xie Y, Zhu Q, Liu Y-G*. 2017. Targeted genome editing in genes and cis-regulatory regions improves qualitative and quantitative traits in crops. Molecular Plant. 10:1368–1370.

23. Shen R, Wang L, Liu X, Wu J, Jin W, Zhao X, Xie X, Zhu Q, Tang H, Li Q, Chen L, Liu Y-G*. 2017. Genomic structural variation-mediated allelic suppression causes hybrid male sterility in rice. Nature Communications. DOI: 10.1038/s41467-017-01400-y.

24. Zhu Q, Yu S, Zeng D, Liu H, Wang H, Yang Z, Xie X, Shen R, Tan J, Li H,Zhao X, Zhang Q, Chen Y, Guo J, Chen L, Liu Y-G *.2017.Development of ‘‘Purple Endosperm Rice’’ by Engineering Anthocyanin Biosynthesis in the Endosperm with a High-Efficiency Transgene Stacking System. Molecular Plant. 10:918–929.

25. Xie X, Ma X, Zhu Q, Zeng D, Li G, Liu Y-G *. 2017.CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing. Molecular Plant.10:1246–1249.

26. Xie Y, Xu P, Huang J, Ma S,Xie X, Tao D, Chen L*, Liu Y-G *. 2017. Interspecific Hybrid Sterility in Rice Is Mediated by OgTPR1 at the S1 Locus Encoding a Peptidase-like Protein. Molecular Plant.10:1137–1140.

27.  Tang H, Zheng X, Li C, Xie X,Chen Y, Chen L,Zhao X, Zheng H, Zhou J,Ye S, Guo J*, Liu Y-G*. 2017. Multi-step formation, evolution, and functionalization of new cytoplasmic male sterility genes in the plant mitochondrial genomes. Cell Research. 27:130-146. doi:10.1038/cr.2016.115.

28.  Ma X, Zhu Q, Chen Y, Liu Y-G*. 2016. CRISPR/Cas9 platforms for genome editing in plants: developments and applications. Molecular Plant. 9:961–974.

29.  Liu W, Xie X, Ma X, Li J, Chen J, Liu Y-G*. 2015. DSDecode: a webbased tool for decoding of sequencing chromatograms for genotyping of targeted mutations. Molecular Plant. 8:1431-1433.

30.  Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H,Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X, Dong Z, Liu Y-G*. 2015. A robust CRISPR/Cas9 system for convenient high-efficiency multiplex genome editing in monocot and dicot plants. Molecular Plant. 8:1274-1284.

31.  Ma X, Chen L, Zhu Q, Chen Y, Liu Y-G*. 2015. Rapid decoding of sequence-specific nuclease-induced heterozygous and biallelic mutations by direct sequencing of PCR products. Molecular Plant. 8:1285-1287.

32.  Tang H, Luo D, Zhou D, Zhang Q, Tian D, Zheng X, Chen L*, Liu Y-G*. 2014. The rice restorer Rf4 for Wild Abortive cytoplasmic male sterility encodes a mitochondrial-localized PPR protein that functions in reduction of WA352 transcripts. Molecular Plant. 7:1497-1500.

33.  Chen L and Liu Y-G*. 2014. Male Sterility and Fertility Restoration in Crops. Annual Review of Plant Biology. 65: 579-606.

34.  Ji C, Li H Chen, L, Xie M, Wang F, Chen Y, Liu Y-G* 2013. A novel rice bHLH transcription factor, DTD, acts coordinately with TDR in controlling tapetum function and pollen development. Molecular Plant. 6(5):1715-1718.

35.  Luo D , Xu H, Liu Z , Guo J, Li H, Chen L, Fang C, Zhang Q, Bai M, Yao N, Wu H, Ji C, Zheng H, Chen Y, Ye S, Li X, Zhao X, Li R, Liu Y-G*. 2013. A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice. Nature Genetics. 45:573-577.

 

发明专利

1. 一种水稻株高株型调节基因OsUBR7及其应用(发明人:刘耀光,郑洋谊,初志战,罗燕秋,张森森,林惠芳,赵秀彩),专利号:ZL202210248417.4(授权日:2023.6.20)

2. 一种植物高效胞嘧啶单碱基编辑器及其构建与应用(刘耀光,祝钦泷,曾栋昌),专利号:ZL202011189809.5(授权日:2022. 08.09)

3. 一种鉴定水稻品种的CAPS分子标记方法及应用(张群宇,刘耀光,周峰,王曼,薛德星,裴蕾,江燕翔,赵秀彩,祝钦泷),专利号:ZL 201710954810.4授权日:2021. 03. 12

4. 一种多基因组装载体系统及其多基因组装方法(刘耀光,祝钦泷),专利号: ZL201710384197.7(授权日:2021. 02.19)

5. 一种水稻mTERF转录终止因子V14基因及其应用(张群宇,刘耀光,周峰,王曼,裴蕾,薛德星,谢敏,江燕翔), 专利号:ZL201710954813.8(授权日:2021.03.12)

6. 一个控制亚非稻杂种不育的基因S1A4及应用(陈乐天,刘耀光,谢勇尧,汤金涛,李小娟,费越),专利号:ZL 201811378142.6授权日:2021. 01. 26

7. 一种创建亚洲栽培稻与非洲栽培稻种间杂种亲和系的方法和应用(陈乐天,刘耀光,谢勇尧,黄健乐), 专利号:ZL201710216552.X(授权日:2020.12.29)

8. 一种短小型小核RNA启动子及其构建方法和在基因组编辑中的应用(刘耀光,郭晶心,祝钦泷,郝雨,宗伍辈),专利号:ZL201911338845.0(授权日:2020. 12.11

9. 一种在作物种子胚乳合成花青素的转基因育种方法(刘耀光,祝钦泷,余穗泽,曾栋昌),专利号:ZL201710368951.8(授权日:2020. 11. 13)

10. OsPSE1基因在调节植物衰老中的应用(陈乐天,刘耀光,吴海斌,韩靖鸾,谢勇尧,王斌,陈远玲),专利号:ZL201510020028.6(授权日:2020.08.07)

11. 一种花药特异表达启动子PV4及其应用(祝钦泷,刘耀光,谭健韬),专利号:ZL201710369461.X授权日:2020. 04.17

12. 一种在作物种子胚乳生产虾青素的转基因育种方法(刘耀光,祝钦泷,曾栋昌,陈乐天),专利号:ZL201610283794.6(授权日:2020. 02.21)

13. 一种调控水稻植株株高的方法(陈远玲,李日清,徐毅炜,陈水福,夏继星,刘耀光,陈乐天,毛润媛,刘鹏),专利号: ZL201510840714.8(授权日:2020.02.04)

14. 一种水稻育性基因SAW1及其应用(祝钦泷,刘耀光,陈乐天,方瑞秋,王斌,陈法铭,韩靖鸾),专利号:ZL20191192671.1(授权日:2019. 11. 28)

15. 一种植物生长负调控顺式元件SE1及在调控植物株高中的应用(陈乐天,谢勇尧,张雅玲,黄建乐,李元元,田庆玮,陈远玲,刘耀光),专利号:ZL201510969303.9(授权日:2019.02.01)

16. 水稻细胞质雄性不育恢复基因及其应用 (刘耀光,唐辉武,罗荡平,陈乐天,张群宇),专利申请号:201410090900.X(授权日2016.12.19)

17. 一种水稻雄性育性可控系的构建方法及其在杂交育种的应用(刘耀光,牛百晓,王平,祝钦泷,林玉茹),专利号:ZL 201210339978.1(授权日:2015.5.20)

18. 一种水稻细胞质雄性不育基因及其应用(刘耀光,刘振兰,徐虹,吴豪,郭晶心,张群宇,叶珊,陈远玲),专利号:ZL 200810167024.0 (授权日:2011.5.18)

19. 水稻杂种花粉育性基因及其应用 (刘耀光,龙云铭,赵利锋,牛白晓,庄楚雄),专利号: ZL200810132341.9 (授权日:2011.3.23)

20. 一种水稻杂种育性基因及其应用 (刘耀光,杨绍华,陈竹锋,庄楚雄,梅曼彤),专利号: ZL200610011976.4(授权日:2009.2.4)

21. 植物细胞质雄性不育恢复基因及其应用 (刘耀光, 王中华, 吴豪, 张群宇),专利号: ZL02152010.0(授权日:2002.11.20)

22. 多基因载体的构建方法与应用 (刘耀光),专利号: ZL02134869.3(授权日:2006.7.12)



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